Tuesday, February 19, 2008

A New Fluorescent collagen probe to use on fresh tissue


Collagen is one the important load-bearing components in the body and the collagen structure has a distinct role in mechanical properties of the tissue. One of the challenges today is to visualize the collagen structure in live tissues or fresh tissues without fixing the tissue. It's clear that fixing methods are invasive and may alter the tissue structure. As an example, in tissue engineering experiments, visualization of changes in collagen three dimensional structure is essential for the understanding of collagen fibrils formation and remodeling.

Fluorescent CNA35 collagen probe developed in the laboratory of macromolecular and organic chemistry, Eindhoven University of Technology, The Netherlands, shows much specificity to collagen compared to other existing fluorescent techniques currently used for collagen visualization in live tissues and shows improved details compared to SHG ( second harmonic generation microscopy). However, in my opinion, the tissue should be relatively thin to let the prob perfuse all over the tissue.

The figure shows a mouse carotid artery, (Up) SHG signal of collagen (green), (down) fluorescence signal of collagen probe (green, CNA35-OG488) in a mouse carotid artery recorded at the same focal position obtained with two-photon laser scanning microscopy (15 μm deep)

reference:
High resolution imaging of collagen organisation and synthesis using a versatile collagen specific probe, Journal of Structural Biology
Volume 159, Issue 3, September 2007, Pages 392-399

6 comments:

suresh said...

Hi Rana

How is the collagen probe injected into the tissue and what is the excitation wavelength and the emission wavelength.

I am working at ocean optics and we have an flouroscent optical probe(like a reflectance probe)

http://www.oceanoptics.com/products/qf600-8.asp


and was interested in doing some experiment to see flouroscence measurement of chicken skin

Rana Rezakhaniha said...

Hi,

I used this prob on excised arterial tissue after incubation in 2 microM of the probe solution over the night.
So, the application of the probe was ex-vivo and no injection needed. The excitation wavelength: 485 nm and the emission is green for the CNAII. For more info, you can take a look at the article mentioned as reference.

Take care,
Rana

xing said...

Hi Rana:
Is it a commerical probe? I want to buy it to do some experiments.
Best regards.
Jiong Xing

xing said...

Hi Rana:
I am a postdoctoral fellow in Research Institute & Department of Radiology,The Methodist Hospital ,Weill Medical College,Cornell University, USA
I want to know whether this is a commercial produce. If it is , How can I buy it? I want to use it to do some experiments
Thank you very much.
Jiong Xing

Rana Rezakhaniha said...

Hi Xing,

I don't think that the probe is commercially available for now. You could contact people of the Eindhoven university to check if you could get some from them.

Anonymous said...

The problem is, when scientists publish in venues that then charge significant dollars for reprints to those who are outside the standard academic flux, the march of progress into commercial and then consumer led directions, is stymied. I must pay to get a copy of your paper ($32.00), is that good for you?